Porcine Conceptus Oct-4 mRNA Expression During Peri-implantation Development
Reproduction in Domestic Animals
OCT-4 is a transcriptional regulator of pluripotent cells throughout embryonic and germ cell lineage development prior to cellular differentiation during murine, bovine and porcine peri-implantation development. In contrast to murine OCT-4 expression, bovine and porcine expression is detected in both the inner cell mass and trophoblast. Delayed down regulation of OCT-4 gene expression in farm species may be a consequence of the lengthened period of peri-implantation. Expression of OCT-4 mRNA has not been characterized during conceptus attachment to the uterine surface in the pig. The objective of the present study was to determine conceptus OCT-4 mRNA expression during porcine peri-implantation development from days 10–17 of gestation. Total RNA was extracted from multiple pools of conceptuses collected on days 10, 12, 13, 15 and 17 of pregnancy. Quantitative RT-PCR was utilized to assay conceptus OCT-4 mRNA synthesis. Day of conceptus development significantly affected (p < 0.001) OCT-4 mRNA expression. Conceptus expression of OCT-4 was greatest on days 10 and 12 of pregnancy being approximately 7.7- and 11.6-fold greater compared to expression on days 15 and 17, respectively. Results from the present study suggest that down regulation of OCT-4 may be critical in trophoblastic expansion and uterine epithelial attachment during establishment of pregnancy in the pig.
Spencer, D., J. Ross, M. D. Ashworth, R. Geisert, L. Rickords 2006. Porcine conceptus Oct-4 mRNA expression during peri-implantation development. Reproduction in Domestic Animals 41: 571-572.