ADVS Faculty Publications Copyright (c) 2013 Utah State University All rights reserved. http://digitalcommons.usu.edu/advs_facpub Recent documents in ADVS Faculty Publications en-us Sun, 19 May 2013 01:38:09 PDT 3600 Evaluation of Immune Modulators, Interferons and Known In Vitro SARS-CoV Inhibitors for Inhibition of SARS-CoV Replication in BALB/c Mice http://digitalcommons.usu.edu/advs_facpub/1140 http://digitalcommons.usu.edu/advs_facpub/1140 Fri, 17 May 2013 10:52:56 PDT Compounds approved for therapeutic use and in vitro inhibitors of severe acute respiratory syndrome coronavirus (SARS-CoV) were evaluated for inhibition in the mouse SARS-CoV replication model. A hybrid interferon, interferon alpha (IFN-alpha) B/D, and a mismatched double-stranded (ds) RNA interferon (IFN) inducer, Ampligen (poly I:poly C124), were the only compounds that potently inhibited virus titres in the lungs of infected mice as assessed by CPE titration assays. When mice were dosed intraperitoneally (i.p.) with IFN-alpha B/D once daily for 3 days beginning 4 h after virus exposure, SARS-CoV replication in the lungs of infected mice was reduced by 1 log10 at 10,000 and 32,000 IU; at the highest dose of 100,000 IU, virus lung titres were below detectable limits. Ampligen used i.p. at 10 mg/kg 4 h prior to virus exposure also reduced virus lung titres to below detectable limits. Nelfinavir, beta-D-N4-hydroxycytidine, calpain inhibitor VI, 3-deazaneplanocin A and Alferon (human leukocyte IFN-alpha-n3) did not significantly reduce lung virus titres in mice. Anti-inflammatory agents, chloroquine, amodiaquin and pentoxifylline, were also inactive in vivo, suggesting that although they may be useful in ameliorating the hyperinflammatory response induced by the virus infection, they will not significantly reduce the replication of the virus, the inducer of inflammatory response. Thus, anti-inflammatory agents may only be useful in treating virus lung infections if used in combination with agents that inhibit virus replication. In summary, the data suggest that induction of IFN by mismatched dsRNA or actual treatment with exogenous IFN-alpha can inhibit SARS-CoV replication in the lungs of mice.

]]> Dale L. Barnard Update on Utah Veterinary Diagnostic Laboratory testing of milk samples including developments in milk pregnancy testing http://digitalcommons.usu.edu/advs_facpub/1139 http://digitalcommons.usu.edu/advs_facpub/1139 Mon, 25 Mar 2013 08:39:13 PDT David J. Wilson SPC in pasteurized milk for calves – effects of milk handling and questions for the future http://digitalcommons.usu.edu/advs_facpub/1138 http://digitalcommons.usu.edu/advs_facpub/1138 Mon, 25 Mar 2013 08:39:12 PDT David J. Wilson et al. Utilization of the new bovine pregnancy test offered at the Utah Veterinary Diagnostic Laboratory http://digitalcommons.usu.edu/advs_facpub/1136 http://digitalcommons.usu.edu/advs_facpub/1136 Mon, 25 Mar 2013 08:39:11 PDT David J. Wilson Influence of season, ambient temperature and time until feeding on bacteria counts in pasteurized milk for dairy calves http://digitalcommons.usu.edu/advs_facpub/1137 http://digitalcommons.usu.edu/advs_facpub/1137 Mon, 25 Mar 2013 08:39:11 PDT David J. Wilson et al. Mycoplasma spp. isolated from bulk tank milk and plans for testing of individual cows http://digitalcommons.usu.edu/advs_facpub/1135 http://digitalcommons.usu.edu/advs_facpub/1135 Mon, 25 Mar 2013 08:39:10 PDT David J. Wilson et al. Test characteristics and multiple demonstration herd application of the DHIA milk Johne’s ELISA http://digitalcommons.usu.edu/advs_facpub/1134 http://digitalcommons.usu.edu/advs_facpub/1134 Mon, 25 Mar 2013 08:39:09 PDT David J. Wilson et al. Reduction of mycoplasma in dairy herds and information on Johne’s disease in dairy herds – 2 surveillance projects in Utah http://digitalcommons.usu.edu/advs_facpub/1133 http://digitalcommons.usu.edu/advs_facpub/1133 Mon, 25 Mar 2013 08:39:09 PDT David J. Wilson An experiment in transmission of M. bovis to naive dairy calves from positive bedding sand http://digitalcommons.usu.edu/advs_facpub/1132 http://digitalcommons.usu.edu/advs_facpub/1132 Mon, 25 Mar 2013 08:39:07 PDT David J. Wilson et al. Environmental mastitis and the dry period http://digitalcommons.usu.edu/advs_facpub/1130 http://digitalcommons.usu.edu/advs_facpub/1130 Mon, 25 Mar 2013 08:39:06 PDT David J. Wilson New findings in mastitis caused by mycoplasma http://digitalcommons.usu.edu/advs_facpub/1131 http://digitalcommons.usu.edu/advs_facpub/1131 Mon, 25 Mar 2013 08:39:06 PDT David J. Wilson Mastitis pathogens from 3755 dairy goats and sheep and farm characteristics in the Northeast US http://digitalcommons.usu.edu/advs_facpub/1129 http://digitalcommons.usu.edu/advs_facpub/1129 Mon, 25 Mar 2013 08:39:05 PDT David J. Wilson Mycoplasma mastitis: statewide prevalence and characteristics of infected dairy herds in Utah http://digitalcommons.usu.edu/advs_facpub/1128 http://digitalcommons.usu.edu/advs_facpub/1128 Mon, 25 Mar 2013 08:39:04 PDT David J. Wilson Mycoplasma in dairy cattle and early results from a voluntary surveillance project in Utah http://digitalcommons.usu.edu/advs_facpub/1127 http://digitalcommons.usu.edu/advs_facpub/1127 Mon, 25 Mar 2013 08:39:03 PDT David J. Wilson Mastitis pathogens from dairy goats and sheep and farm characteristics 1993-2004 http://digitalcommons.usu.edu/advs_facpub/1126 http://digitalcommons.usu.edu/advs_facpub/1126 Mon, 25 Mar 2013 08:39:02 PDT David J. Wilson Measures of and factors affecting raw milk quality http://digitalcommons.usu.edu/advs_facpub/1124 http://digitalcommons.usu.edu/advs_facpub/1124 Mon, 25 Mar 2013 08:39:01 PDT David J. Wilson Introduction and services available from new dairy extension veterinarian http://digitalcommons.usu.edu/advs_facpub/1125 http://digitalcommons.usu.edu/advs_facpub/1125 Mon, 25 Mar 2013 08:39:01 PDT David J. Wilson Comparison of J-5 vaccinates and controls following naturally occurring cases of Clinical mastitis http://digitalcommons.usu.edu/advs_facpub/1123 http://digitalcommons.usu.edu/advs_facpub/1123 Mon, 25 Mar 2013 08:39:00 PDT David J. Wilson et al. QMPS farm survey data January-November 2003 http://digitalcommons.usu.edu/advs_facpub/1122 http://digitalcommons.usu.edu/advs_facpub/1122 Mon, 25 Mar 2013 08:38:59 PDT David J. Wilson et al. Managing mycoplasma using risk assessment http://digitalcommons.usu.edu/advs_facpub/1120 http://digitalcommons.usu.edu/advs_facpub/1120 Mon, 25 Mar 2013 08:38:58 PDT David J. Wilson