Chondrostereum purpureum as a biological control agent in forest vegetation management. III. Infection survey of a national field trial
Contribution to Book
Proceedings of the third International Conference on Forest Vegetation Management
A nationally coordinated field trial was established in the summer of 1995 to assess the utility of Chondrostereum purpureum (Pers. ex Fr.) Pouzar as a biocontrol agent for several weedy deciduous species. In this study, C. purpureum infection of cut stumps was surveyed 4 months following applications of the biocontrol agent in New Brunswick and Ontario field trials. The use of diagnostic molecular genetic markers to detect and identify C. purpureum was compared with morphological identification methods. Samples of trembling aspen (Populus tremuloides Michx.) and red maple (Acer rubrum L.) from the New Brunswick trials confirmed infection with the biocontrol isolates of C. purpureum, and no cross contamination of treatment applications was detected. Analyses of recovered fungal samples from aspen and speckled alder (Alnus rugosa (Du Roi) Spreng.) in Ontario also confirmed the success of biocontrol infection. The percentage of C. purpureum infected stumps differed between hosts in the Ontario trials. An average of 84% of speckled alder stems and 54% of aspen stems were estimated to have been infected by the applied isolates of C. purpureum. Given a sufficient number of samples, this methodology can provide an early indication of successful stump infection by C. purpureum, a prerequisite for effective weed biocontrol.
Becker, E.M.; Ball, L.A.; Dumas, M.T.; Pitt, D.G.; Wall, R.E.; and Hintz, W.E., "Chondrostereum purpureum as a biological control agent in forest vegetation management. III. Infection survey of a national field trial" (1999). Aspen Bibliography. Paper 1119.