Aspen Bibliography

Inheritance and linkage of allozymes and restriction fragment length polymorphisms in trembling aspen

Document Type

Article

Journal/Book Title/Conference

Journal of Heredity

Volume

84

Issue

5

First Page

419

Last Page

424

Publication Date

1993

Abstract

Allozyme variants in leaf tissue of the parents and progeny of five full-sIb families of trembling aspen (Populus tremuloides; n = 19) were examined for nine enzyme systems. We consistently resolved allozymes of 14 loci, among which eight were polymorphic (Aco-1, Aco-2, Got-3, Per-1, Pgm-1, 6Pg-2, Pgi-2, and Skdh) and six were monomorphic (Got-1, Got-2, Mdh-1, Mdh-2, Pgl-1, and Sod). No significant deviation from the expected segregation ratios was observed for these allozyme loci. The results of joint two-locus segregation analyses indicated no linkage between 12 testable allozyme locus pairs. In one of these full-sib families of 93 progeny, we examined inheritance and linkage of four segregating allozyme loci and 75 RFLP loci corresponding to clones from an aspen Pst I genomic DNA library. None of the segregation ratios of the RFLP loci deviated significantly from those expected. We have constructed a linkage map that incorporates 54 RFLP and three allozyme loci comprising 14 linkage groups and covering 664 cM of the genome.

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