Bioconversion of acid-hydrolyzed poplar hemicellulose to acetic acid by Clostridium thermoaceticum

Authors

J.E. Brownell
J.P. Nakas

Document Type

Article

Journal/Book Title/Conference

Journal of Industrial Microbiology

Volume

7

Issue

1

First Page

1

Last Page

6

Publication Date

1991

Abstract

Clostridium thermoaceticum was used to ferment carbohydrate released from pretreated oat splet xylan and hemicellulose isolated from hybrid poplar. Hydrolysis with dilute sulfuric acid (2.5% (v/v) for oat spelt xylan and 4.0% (v/v) for poplar hemicellulose) at 100°C for 60 min was found to release the highest concentration of fermentable substrate.C. thermoaceticum, when grown in non-pH controlled batch culture at 55°C under a headspace of 100% CO2, typically produced 14gl−1 acetic acid during a 48 h fermentation in medium containing 2% xylose. In fed-batch fermentations this organism was able to produce 42gl−1 acetic acid after 116h when the concentration of xylose was maintained at approximately 2% and the pH was controlled at 7.0.

Recommended Citation

Brownell, J. E. and Nakas, J. P. 1991. Bioconversion of acid-hydrolyzed poplar hemicellulose to acetic acid by Clostridium thermoaceticum. Journal of Industrial Microbiology. 7 (1): 1-6.