Chemistry and Biochemistry Faculty Publications Copyright (c) 2013 Utah State University All rights reserved. http://digitalcommons.usu.edu/chem_facpub Recent documents in Chemistry and Biochemistry Faculty Publications en-us Wed, 06 Mar 2013 01:33:30 PST 3600 Investigation of Antibacterial Mode of Action for Traditional and Amphiphilic Aminoglycosides http://digitalcommons.usu.edu/chem_facpub/455 http://digitalcommons.usu.edu/chem_facpub/455 Mon, 04 Mar 2013 08:20:18 PST Aminoglycoside represents a class of versatile and broad spectrum antibacterial agents. In an effort to revive the antibacterial activity against aminoglycoside resistant bacteria, our laboratory has developed two new classes of aminoglycoside, pyranmycin and amphiphilic neomycin (NEOF004). The former resembles the traditional aminoglycoside, neomycin. The latter, albeit derived from neomycin, appears to exert antibacterial action via a different mode of action. In order to discern that these aminoglycoside derivatives have distinct antibacterial mode of action, RNA-binding affinity and fluorogenic dye were employed. These studies, together with our previous investigation, confirm that pyranmycin exhibit the traditional antibacterial mode of action of aminoglycosides by binding toward the bacterial rRNA. On the other hand, the amphiphilic neomycin, NEOF004 disrupts the bacterial cell wall. In a broader perspective, it verifies that structurally modified neomycin can exert different antibacterial mode of action leading to the revival of activity against aminoglycoside resistant bacteria.

]]> Venkatareddy Udumula et al. Phosphoryl and Sulfuryl Transfer http://digitalcommons.usu.edu/chem_facpub/454 http://digitalcommons.usu.edu/chem_facpub/454 Tue, 02 Oct 2012 13:16:23 PDT Tiago A.S. Brandão et al. Secondary Isotope Effects http://digitalcommons.usu.edu/chem_facpub/453 http://digitalcommons.usu.edu/chem_facpub/453 Tue, 02 Oct 2012 13:16:22 PDT Alvan C. Hengge Phosphoryl Transfer Reactions http://digitalcommons.usu.edu/chem_facpub/452 http://digitalcommons.usu.edu/chem_facpub/452 Tue, 02 Oct 2012 13:16:21 PDT Alvan C. Hengge Kinases http://digitalcommons.usu.edu/chem_facpub/451 http://digitalcommons.usu.edu/chem_facpub/451 Tue, 02 Oct 2012 13:16:21 PDT Alvan C. Hengge Phosphatases http://digitalcommons.usu.edu/chem_facpub/450 http://digitalcommons.usu.edu/chem_facpub/450 Tue, 02 Oct 2012 13:16:20 PDT Alvan C. Hengge Insights from Heavy-Atom Isotope Effects on Phosphoryl and Thiophosphoryl Transfer Reactions http://digitalcommons.usu.edu/chem_facpub/449 http://digitalcommons.usu.edu/chem_facpub/449 Tue, 02 Oct 2012 13:16:19 PDT Alvan C. Hengge Transfer of the PO<sub>3</sub><sup>2-</sup> Group http://digitalcommons.usu.edu/chem_facpub/448 http://digitalcommons.usu.edu/chem_facpub/448 Tue, 02 Oct 2012 13:16:18 PDT Alvan C. Hengge The molecular details of WPD-loop movement differ in the protein-tyrosine phosphatases YopH and PTP1B http://digitalcommons.usu.edu/chem_facpub/447 http://digitalcommons.usu.edu/chem_facpub/447 Tue, 02 Oct 2012 13:16:17 PDT Tiago A.S. Brandão et al. Insights into the Phosphoryl Transfer Mechanism of Cyclin-Dependent Protein Kinases from ab Initio QM/MM Free-Energy Studies http://digitalcommons.usu.edu/chem_facpub/446 http://digitalcommons.usu.edu/chem_facpub/446 Tue, 02 Oct 2012 13:16:17 PDT Gregory K. Smith et al. The Divalent Metal Ion in the Active Site of Uteroferrin Modulates Substrate Binding and Catalysis http://digitalcommons.usu.edu/chem_facpub/445 http://digitalcommons.usu.edu/chem_facpub/445 Tue, 02 Oct 2012 13:16:16 PDT Natasa Mitic et al. Insights into the Reaction of Proteintyrosine Phosphatase 1B: Crystal Structures for Transition State Analogs of Both Catalytic Steps http://digitalcommons.usu.edu/chem_facpub/444 http://digitalcommons.usu.edu/chem_facpub/444 Tue, 02 Oct 2012 13:16:14 PDT Tiago A.S. Brandão et al. Active-Site Dynamics of SpvC Virulence Factor from Salmonella typhimurium and Density Functional Theory Study of Phosphothreonine Lyase Catalysis http://digitalcommons.usu.edu/chem_facpub/443 http://digitalcommons.usu.edu/chem_facpub/443 Tue, 02 Oct 2012 13:16:13 PDT Gregory K. Smith et al. Mechanism and Transition State Structure of Aryl Methylphosphonate Esters Doubly Coordinated to a Dinuclear Cobalt(III) Center http://digitalcommons.usu.edu/chem_facpub/442 http://digitalcommons.usu.edu/chem_facpub/442 Tue, 02 Oct 2012 13:16:12 PDT Guoqiang Feng et al. Impaired acid catalysis by mutation of a protein loop hinge residue in a YopH mutant revealed by crystal structures http://digitalcommons.usu.edu/chem_facpub/441 http://digitalcommons.usu.edu/chem_facpub/441 Tue, 02 Oct 2012 13:16:11 PDT T. A.S. Brandão et al. An Altered Transition State for the Reaction of an RNA Model Catalyzed by a Dinuclear Zinc(II) Catalyst http://digitalcommons.usu.edu/chem_facpub/440 http://digitalcommons.usu.edu/chem_facpub/440 Tue, 02 Oct 2012 13:16:10 PDT T. Humphry et al. Substrate-Promoted Formation of a Catalytically Competent Binuclear Center and Regulation of Reactivity in a Glycerophosphodiesterase from Enterobacter aerogenes http://digitalcommons.usu.edu/chem_facpub/439 http://digitalcommons.usu.edu/chem_facpub/439 Tue, 02 Oct 2012 13:16:09 PDT K. S. Hadler et al. Mechanistic Study of Protein Phosphatase-1 (PP1), A Catalytically Promiscuous Enzyme http://digitalcommons.usu.edu/chem_facpub/438 http://digitalcommons.usu.edu/chem_facpub/438 Tue, 02 Oct 2012 13:16:08 PDT C. McWhirter et al. The Effects of Sulfur Substitution for the Nucleophile and Bridging Oxygen Atoms in Reactions of Hydroxyalkyl Phosphate Esters http://digitalcommons.usu.edu/chem_facpub/437 http://digitalcommons.usu.edu/chem_facpub/437 Tue, 02 Oct 2012 13:16:07 PDT Subashree Iyer et al. The Use of Isotopes in the Study of Reactions of Acyl, Phosphoryl, and Sulfuryl Esters http://digitalcommons.usu.edu/chem_facpub/436 http://digitalcommons.usu.edu/chem_facpub/436 Tue, 02 Oct 2012 13:16:06 PDT R. H. Hoff et al.