Several commercially available fluoresceinisothiocyanate and rhodamine isothiocyanateconjugated plant lectins have been applied to cereal and oilseed tissues to permit identification and localization of specific structures and carbohydrates by fluorescence microscopy . Ulex europeaus Agglutinin I (UEAl) and Ricinis communis Agglutinin I (RCA I) showed specificity for the amyloids in rapeseed cotyledonary cell walls . Wheat Germ Agglutinin (WGA) bound to rapeseed coat mucilage, as well as fungal hyphae in infected wheat . Lens culinaris Agglutinin (LCA) bound only to starch in cereal sections, and at higher magnifications of isolated starch granules , the annular structure was clearly visible.
Miller, S. Shea; Yiu, S. H.; Fulcher, R. G.; and Altosaar, I.
"Preliminary Evaluation of Lectins as Fluorescent Probes of Seed Structure and Composition,"
Food Structure: Vol. 3
, Article 5.
Available at: http://digitalcommons.usu.edu/foodmicrostructure/vol3/iss2/5