Preliminary Evaluation of Lectins as Fluorescent Probes of Seed Structure and Composition

Authors

S. Shea Miller
S. H. Yiu
R. G. Fulcher
I. Altosaar

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This work is licensed under a Creative Commons Attribution 4.0 License.

Abstract

Several commercially available fluoresceinisothiocyanate and rhodamine isothiocyanateconjugated plant lectins have been applied to cereal and oilseed tissues to permit identification and localization of specific structures and carbohydrates by fluorescence microscopy . Ulex europeaus Agglutinin I (UEAl) and Ricinis communis Agglutinin I (RCA I) showed specificity for the amyloids in rapeseed cotyledonary cell walls . Wheat Germ Agglutinin (WGA) bound to rapeseed coat mucilage, as well as fungal hyphae in infected wheat . Lens culinaris Agglutinin (LCA) bound only to starch in cereal sections, and at higher magnifications of isolated starch granules , the annular structure was clearly visible.

Recommended Citation

Miller, S. Shea; Yiu, S. H.; Fulcher, R. G.; and Altosaar, I. (1984) "Preliminary Evaluation of Lectins as Fluorescent Probes of Seed Structure and Composition," Food Structure: Vol. 3: No. 2, Article 5.
Available at: https://digitalcommons.usu.edu/foodmicrostructure/vol3/iss2/5