Food Structure


Fluorescence microscopy was used to characterize the mature car yaps is of Sorghum bicolor (L.) Moench . Acid Fuchsin, a protein specific dye used in bright field microscopy, caused protein bodies and matrix in the sorghum endosperm to fluoresce. ANS (8-ani lino-1- naphthal e ne sulfonic acid) also caused the protein bodi es and matrix in the endosperm to fluoresce. Varietal differences in endosperm protein dist r ibution were evident when viewed after sta · ning with Acid Fuchsin . Nile Blue A caused fl uorescence in neutral lipids such as those in :he lipid bodies in the aleurone and scutellum of sorg hum. Nile Blue A also caused fluoresce nce in two cuticular l ayers, one on the out s i de of the sorghum ker nel and the other next to t he aleurone layer . The bright f ield Sudan Ill and IV stai ns were used t o confirm the presence of these cutic ular layers. After staining with both Ehrlic h 's reagent and dimethylami nocinnamaldehyde, fluoresc ence due to aromat ic ami nes was not observed in the a 1 eu rone of sorghum. After staining with diphenylborinic acid, a marker for flavonoids, fluorescence in the aleurone cell walls was observed. Periodi c acid/Sch iff ' s reagent was used to view starch in the sorghum endosperm. Acrlflavine- HCl produced fluorescence in phytin granules in the scutellum of sorghum; no fluorescence was observed in the aleurone. When treated with cyanogen bromide and either barbituric acid or paminobenzoic acid, nicotinic acid deposits were detected in inclusions in both the a leurone and scutellum of sorghum.

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