Food Structure
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Abstract
Tissue damage creates numerous problems for the microscopic examination of resting peanut (Arachis hypogaea L. cv . Florunner) seed physical structure. This paper presents a method to deal with specific problems encountered in light microscopy (LM) and scanning electron microscopy (SEM) of drought - induced damage of peanut tissue . Major findings include: (1) improved SEM imaging through reduction of charging by increasing coating thickness from 25 nm to 30 nm; (2) improved stain affinity for LM tissue preparations by decreasing osmium tetroxide fixation time from 2 hours to 1 hour; and (3) im proved tissue fixation for LM and SE M by application of a modified Karnovsky fixative (glutaraldehyde / formaldehyde combination) which proved more successful than glutaraldehyde alone for tissue preservation.
Recommended Citation
Young, Clyde T. and Schadel, William E.
(1989)
"A Method for Light and Scanning Microscopy of Drought-Induced Damage of Resting Peanut Seed Tissue,"
Food Structure: Vol. 8:
No.
2, Article 11.
Available at:
https://digitalcommons.usu.edu/foodmicrostructure/vol8/iss2/11