Title

Inactivation of Clostridial Ferredoxin and Pyruvate-Ferredoxin Oxidoreductase by Sodium Nitrite

Document Type

Article

Journal/Book Title/Conference

Applied and Environmental Microbiology

Volume

53

Issue

3

Publisher

American Society for Microbiology

Publication Date

1987

First Page

549

Last Page

552

Abstract

Clostridial ferredoxin and pyruvate-ferredoxin oxidoreductase activity was investigated after in vitro or in vivo treatment with sodium nitrite. In vitro treatment of commercially available Clostridium pasteurianum ferredoxin with sodium nitrite inhibited ferredoxin activity. Inhibition of ferredoxin activity increased with increasing levels of sodium nitrite. Ferredoxin was isolated from normal C. pasteurianum and Clostridium botulinum cultures and from cultures incubated with 1,000 micrograms of sodium nitrite per ml for 45 min. The activity of in vivo nitrite-treated ferredoxin was decreased compared with that of control ferredoxin. Pyruvate-ferredoxin oxidoreductase isolated from C. botulinum cultures incubated with 1,000 micrograms of sodium nitrite per ml showed less activity than did control oxidoreductase. It is concluded that the antibotulinal activity of nitrite is due at least in part to inactivation of ferredoxin and pyruvate-ferredoxin oxidoreductase.

Comments

Originally published by The American Society for Microbiology. Abstract and full text available through remote link.