Title of Oral/Poster Presentation

Rapid prototyping of paper-based micro-device using wax and material printer for low-cost portable glucose assay

Presenter Information

Han ZhangFollow

Class

Article

Graduation Year

2018

College

College of Engineering

Department

Biological Engineering Department

Faculty Mentor

Anhong Zhou

Presentation Type

Oral Presentation

Abstract

Here we present a simple and low-cost production method to generate paper-based microfluidic devices for portable glucose assay. The device consists of two parts: sample loading zone and bioassay zone. Black wax was printed on Whatman filter paper and baked at 150ºC for 2mins in order to build up hydrophobic boundary. DMP 2831 material printer was applied to print chromogenic substrate (3,3’,5,5’-Tetramethylbenzidine) enzyme mixture (glucose oxidase (GOx) and Horseradish peroxidase (HRP)) onto the bioasssay zone. Printing method provides more homogeneous coating than dropping on method. The ratio of GOx:HRP concentration is 20ug/ml:100ug/ml. The linear range is from 0.02mg/ml to 1mg/ml. Three different type of filter papers (Watman No.1, 4 and 5) were evaluated. The known and unknown concentration of glucose solutions were tested and compared by our device and other methods. The device is of low cost; fast testing; high stability and reliability. Our device provide a portable platform for blood and urea glucose concentration analysis.

Location

Room 208

Start Date

13-4-2017 10:30 AM

End Date

13-4-2017 11:45 AM

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Apr 13th, 10:30 AM Apr 13th, 11:45 AM

Rapid prototyping of paper-based micro-device using wax and material printer for low-cost portable glucose assay

Room 208

Here we present a simple and low-cost production method to generate paper-based microfluidic devices for portable glucose assay. The device consists of two parts: sample loading zone and bioassay zone. Black wax was printed on Whatman filter paper and baked at 150ºC for 2mins in order to build up hydrophobic boundary. DMP 2831 material printer was applied to print chromogenic substrate (3,3’,5,5’-Tetramethylbenzidine) enzyme mixture (glucose oxidase (GOx) and Horseradish peroxidase (HRP)) onto the bioasssay zone. Printing method provides more homogeneous coating than dropping on method. The ratio of GOx:HRP concentration is 20ug/ml:100ug/ml. The linear range is from 0.02mg/ml to 1mg/ml. Three different type of filter papers (Watman No.1, 4 and 5) were evaluated. The known and unknown concentration of glucose solutions were tested and compared by our device and other methods. The device is of low cost; fast testing; high stability and reliability. Our device provide a portable platform for blood and urea glucose concentration analysis.