Title of Oral/Poster Presentation

Putting an End to Endotoxins

Presenter Information

Richard DeckerFollow

Class

Article

Graduation Year

2018

College

College of Engineering

Department

Biological Engineering Department

Faculty Mentor

Randy Lewis

Presentation Type

Poster Presentation

Abstract

Spider silk has mechanical properties that surpass most man-made materials due to a combination of strength and extensibility. By using synthetic spider silks, these mechanical properties can be tailored to a large variety of uses, ranging from tissue regeneration matrices to medical device coatings. Some of the organisms that have been used for large scale synthetic silk production include E. coli, plants, and goats.

Endotoxins, also known as lipopolysaccarhides (LPS), are heat resistant molecules present in the outer membrane of gram-negative bacteria that illicit strong immune responses in mammals in large doses.Endotoxins are nearly everywhere, but they are only harmful above certain levels and they are typically not harmful when ingested.

Although native spider silk is generally considered to be biocompatible, preliminary tests have shown that the synthetic silks contain a high level of endotoxins. These endotoxins could lead to an inflammatory response when the silk is introduced into a mammalian host. In order to use synthetic spider silk for the many proposed medical applications, it is necessary to remove these endotoxins to prevent any negative immune responses.

Location

South Atrium

Start Date

13-4-2017 1:30 PM

End Date

13-4-2017 2:45 PM

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Apr 13th, 1:30 PM Apr 13th, 2:45 PM

Putting an End to Endotoxins

South Atrium

Spider silk has mechanical properties that surpass most man-made materials due to a combination of strength and extensibility. By using synthetic spider silks, these mechanical properties can be tailored to a large variety of uses, ranging from tissue regeneration matrices to medical device coatings. Some of the organisms that have been used for large scale synthetic silk production include E. coli, plants, and goats.

Endotoxins, also known as lipopolysaccarhides (LPS), are heat resistant molecules present in the outer membrane of gram-negative bacteria that illicit strong immune responses in mammals in large doses.Endotoxins are nearly everywhere, but they are only harmful above certain levels and they are typically not harmful when ingested.

Although native spider silk is generally considered to be biocompatible, preliminary tests have shown that the synthetic silks contain a high level of endotoxins. These endotoxins could lead to an inflammatory response when the silk is introduced into a mammalian host. In order to use synthetic spider silk for the many proposed medical applications, it is necessary to remove these endotoxins to prevent any negative immune responses.