Title of Oral/Poster Presentation

Identifying Changes in C2C12 Cells from Radiation and Zero Gravity Effects using Raman Spectroscopy

Presenter Information

Cameron ZabriskieFollow

Class

Article

Graduation Year

2017

College

College of Engineering

Department

Biological Engineering Department

Faculty Mentor

Dr. Elizabeth Vargis

Presentation Type

Poster Presentation

Abstract

Raman spectroscopy is a promising tool that can be used to identify changes in cells due to radiation or zero gravity. Humans continue to travel farther into space, exposing themselves to the harmful effects of radiation and zero gravity. Many methods are being explored at the biological level to reduce the harmful effects of space travel, and to assess these methods, powerful devices are needed to detect the changes to cells after exposure due to the damaging effects of space. For many years, Raman spectroscopy has been used to analyze the interactions of the bonds of molecules within cells. In this work, Raman spectroscopy has been used on C2C12 mouse muscle cells exposed to both radiation and simulated zero gravity. This technique shows a difference between the Raman spectra of cells exposed to microgravity and of cells exposed to radiation. These cells can then be differentiated from each other as well as a control group that has not been exposed to either condition. This analysis can then be used to provide meaningful insights for researchers aiming to reduce the harmful effects of space travel.

Location

North Atrium

Start Date

13-4-2017 1:30 PM

End Date

13-4-2017 2:45 PM

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Apr 13th, 1:30 PM Apr 13th, 2:45 PM

Identifying Changes in C2C12 Cells from Radiation and Zero Gravity Effects using Raman Spectroscopy

North Atrium

Raman spectroscopy is a promising tool that can be used to identify changes in cells due to radiation or zero gravity. Humans continue to travel farther into space, exposing themselves to the harmful effects of radiation and zero gravity. Many methods are being explored at the biological level to reduce the harmful effects of space travel, and to assess these methods, powerful devices are needed to detect the changes to cells after exposure due to the damaging effects of space. For many years, Raman spectroscopy has been used to analyze the interactions of the bonds of molecules within cells. In this work, Raman spectroscopy has been used on C2C12 mouse muscle cells exposed to both radiation and simulated zero gravity. This technique shows a difference between the Raman spectra of cells exposed to microgravity and of cells exposed to radiation. These cells can then be differentiated from each other as well as a control group that has not been exposed to either condition. This analysis can then be used to provide meaningful insights for researchers aiming to reduce the harmful effects of space travel.