Title

Designing an Artificial Tendon/Graft Derived from Silkworm Silk and Synthetic Spider Silk With Respect to Structure, Mechanical Properties, Biocompatibility, and Attatchment

Document Type

Presentation

Journal/Book Title/Conference

USU Student Showcase

Publication Date

4-2014

Faculty Mentor

Randy Lewis

Abstract

The Achilles tendon is one of the most commonly damaged tendons in the human body (Hansen et al., 2012). Current surgical and therapeutic techniques require extended recovery time and the tendon is rarely returned to its original healthy state. For athletes in particular, strenuous rehabilitation is required to return from a serious injury like a ruptured Achilles tendon (Rosso et al., 2013). Despite the high amount of sources for grafts to aid in surgical repair, all of the options have drawbacks such as availability, immune response, and/or poor mechanical properties. Spider silk has the potential to enhance current Achilles tendon repair techniques resulting in shorter patient recovery times and improved tendon strength and mobility after repair by allowing for early movement of the damaged tendon, which prevents scar formation and promotes growth (James et al., 2008). For an artificial tendon to be successful, it must have a high enough tensile strength and a modulus that is equal to the native tendon. The desired properties, yield strength and stiffness, of a human Achilles tendon have been matched using a transgenic silkworm silk device made of 100 cords, built from single fibers. A twisting method was developed to lower the stiffness of the device to accurately match that of a natural tendon. Biocompatibility studies have been performed on various types of silk threads by growing Chinese Hamster Ovary (CHO) cells on their surfaces in vitro. Under optical microscopy, transgenic silkworm silk without sericin was found to be biocompatible when compared with the positive control of native silkworm silk without sericin. Existing attachment methods have been investigated, which are compatible with a final transgenic silkworm silk device.

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