Cloning and Expression for the Future Characteriz Action of the Air2 Protein
USU Student Showcase
Air2 is a eukaryotic protein involved in multiple biological processes including protein-protein interactions as well as RNA binding. Air2 plays a critical role in RNA quality control and also helps regulate post-translational modification of various proteins. Although previous studies have revealed information regarding Air2's roles within a cell, the molecular and structural basis for Air2 function is unclear. Using a codon-optimized version of the Air2 gene, various constructs were created that improved the expression and solubility of Air2. Additionally a co-expression complex of Air2 with a PRMTI mutant, K13S, was made to obtain the Air2 protein with a native binding partner. It was hypothesized that the co-expression of these two proteins together would lead to the creation of a complex increasing both the stability, expression, and activity of each. These experimental efforts will greatly contribute to the biochemical and structural studies necessary to achieve a better functional understanding of Air2 and thus lead to the improved future characterization of this protein.
Frampton, Emily, "Cloning and Expression for the Future Characteriz Action of the Air2 Protein" (2014). USU Student Showcase. Student Showcase. Paper 56.
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