Title

In Vitro Propagation of the Endangered Virginia Roundleaf Birch (Betula uber [Ashe] Fern.) Using Dormant Buds

Document Type

Article

Journal/Book Title/Conference

36

Volume

3

Publication Date

1990

First Page

842

Last Page

846

Abstract

Dormant buds collected from greenhouse and field-grown trees of Betula uber (Ashe) Fern., the endangered Virginia round-leaf birch, were used for micropropagation. A nutrient medium containing the mineral salts of Murashige and Skoog (1962) supplemented with organic nutrients and growth regulators supported bud break, elongation, and limited multiplication. Shoots were 2-3 cm in height with 3-4 leaves in four weeks. They developed roots in vivo in 4-6 weeks in peat pellets under high humidity conditions with 16 h photoperiod at a temperature of 20°C.