Aspen Bibliography

Changes in peroxidase activity, auxin level and ethylene production during root formation by poplar shoots raised in vitro

J.E. Hausman

Document Type

Article

Journal/Book Title/Conference

Plant Growth Regulation

Volume

13

Issue

3

First Page

263

Last Page

268

Publication Date

1993

Abstract

Shoots of poplar (Populus tremula × P. tremuloïdes) were multiplied in vitro and rooted on a rooting medium in the presence of NAA. No rooting occurred in the absence of exogenous auxin. A peak of soluble peroxidase activity, which corresponded to a decrease in the free IAA level in the shoots, preceded rooting These events were considered as corresponding to the initiative phase of rooting. They are preceded by a peak in free IAA activity which might initiate the inductive phase of the rooting process. A burst of ethylene production was measured in both rooting and non-rooting shoots, but the ethylene peak from rooting shoots appeared earlier and was higher. The use of ACC indicated that the exogenous auxin might have enhanced ACC-synthetase activity.

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