A transcriptional roadmap to wood formation
Proceedings of the national academy of sciences, usa [proc. Natl. Acad. Sci. Usa]
The large vascular meristem of poplar trees with its highly organized secondary xylem enables the boundaries between different developmental zones to be easily distinguished. This property of wood-forming tissues allowed us to determine a unique tissue-specific transcript profile for a well defined developmental gradient. RNA was prepared from different developmental stages of xylogenesis for DNA microarray analysis by using a hybrid aspen unigene set consisting of 2,995 expressed sequence tags. The analysis revealed that the genes encoding lignin and cellulose biosynthetic enzymes, as well as a number of transcription factors and other potential regulators of xylogenesis, are under strict developmental stage-specific transcriptional regulation.
Hertzberg, M.; Aspeborg, H.; Schrader, J.; Andersson, A.; Erlandsson, R.; Blomqvist, K.; Bhalerao, R.; Uhlen, M.; Teeri, T.T.; Lundeberg, J.; Sundberg, B.; Nilsson, P.; and Sandberg, G., "A transcriptional roadmap to wood formation" (2001). Aspen Bibliography. Paper 608.