Journal of the Kansas Entomological Society
Dufour's glands from inactive and active Osmia cornifrons were studied using light and electron microscopy. These glands are very similar in both classes of bees. Each gland consists of a flattened sac of simple epithelial cells bordering a cuticulated lumen with an underlying subcuticular space. Each epithelial cell contains a single nucleus (with several nucleoli), a few short microvilli, numerous elements of rough surfaced endoplasmic reticulum, many mitochondria, sparse Golgi bodies, a few microtubules, and, rarely, a myelin figure. The basal surface of each cell is covered by a basal lamina composed of two distinct layers. Septate junctions occur between cells. Associated visceral muscle cells contain atypical actin and myosin myofilaments with larger than usual diameters and without conventional filament disposition patterns. These cells also contain a transverse tubule system, A-and I-bands, dyads, and Z-lines. Unmyelinated nerve processes, containing empty and full neurosecretory vesicles, are located adjacent to some of the muscles.
Barrows, Edward M.; Chapman, George B.; Zenel, James E.; and Blake, Andrea S., "Ultrastructure of Dufour's Glands in Active and Inactive Horn-faced Bees, Osmia cornifrons (Hymenpotera: Megachilidae)" (1986). Ba. Paper 56.
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