The Proceedings of the Royal Entomological Society of London
MUSSBICHLER (1952) and others have shown that provided young bees are fed on a diet containing carbohydrate and sufficient protein, or on a pure carbohydrate diet after they have eaten sufficient protein, their ovaries develop when no queen is present. Ovary development does not appear to be possible unless protein is available to the bees concerned either in their diet or as reserves within their own bodies.
Butler, C. G., "Some Further Observations on the Nature of "Queen Substance" and of its Role in the Organisation of a Honey-bee (Apis Mellifera) Community." (1956). Bu. Paper 50.
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