Document Type

Article

Author ORCID Identifier

Muhammad Waseem Khan https://orcid.org/0000-0003-3304-9735

Hayat Ullah https://orcid.org/0009-0003-7514-5722

Journal/Book Title/Conference

Applied Microbiology

Volume

5

Issue

1

Publisher

MDPI AG

Publication Date

2-17-2025

Journal Article Version

Version of Record

First Page

1

Last Page

19

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Abstract

Cellulosic biomass is considered an important and sustainable source of renewable energy, which needs a complex mixture of different enzymes for its degradation. After amylase, cellulases are the second most important enzymes, gain more importance due to their broad range of applications at the industrial level, and are considered more economical and environmentally friendly; researchers have focused more on the production of cellulase with its higher expression rate and low cost. Pichia pastoris, a methylotrophic yeast strain, has a more effective and well-established system for the production of heterologous proteins, particularly for industrial enzymes. Moreover, its readily achievable high-density fermentation, high capacity for protein secretion, tractable genetic modifications, typical post-transcriptional modifications, and strong regulated promoters makes it superior to other expression systems. In this review, we address the P. pastoris expression system including protein expression platforms, plasmids, and cellular metabolism quantification as a potential candidate for heterologous protein production, particularly for cellulase enzymes.

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