Document Type

Article

Author ORCID Identifier

Thuy Tien T. Lindsay https://orcid.org/0000-0001-6890-758X

Joseph P. Rinehart https://orcid.org/0000-0002-4794-5226

Karen M. Kapheim https://orcid.org/0000-0002-8140-7712

James P. Strange https://orcid.org/0000-0002-9612-6868

Journal/Book Title/Conference

Journal of Applied Entomology

Volume

148

Issue

6

Publisher

Wiley-Blackwell Verlag GmbH

Publication Date

5-3-2024

Journal Article Version

Version of Record

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

First Page

712

Last Page

722

Abstract

Bumble bees are the primary commercial pollinators for greenhouse-grown crops and managing them requires insight into the Bombus life cycle. However, some aspects of the life cycle are not fully understood. Once mated, adult Bombus gynes can be stored for a short term at a constant low temperature (CLT). However, survival rates for some species are low during CLT, particularly when storage periods exceed a month. To address this, we tested the hypothesis that Bombus gynes stored in a fluctuating thermal regime (FTR) have increased survival compared to gynes stored in CLT. Specifically, the FTR had a baseline temperature of 2°C, with a daily fluctuation to 20°C relative to the CLT of 2°C with no warming pulse. We tested the effects of FTR on gyne survival and post-cold storage lipid content in three species used for commercial pollination in North America: B. vosnesenskii, B. huntii and B. impatiens. We further assessed how additional factors influence cold storage survival, including gyne body weight, body size and colony age. Survival responses varied among species. Bombus vosnesenskii and B. huntii had a lower survival rate in FTR compared to CLT, but B. impatiens gynes had a significantly higher survival rate in FTR. We did not observe significant effects of FTR on lipid stores for any species, but survival increased with an increasing initial weight prior to cold storage. Moreover, gynes produced later in the colony life cycle had lower body weight and decreased survival compared to early emerging gynes. Our study provides insight into how cold storage conditions interact with colony and gyne characteristics to influence captive overwintering survival. Our findings have economic applications for improving commercial rearing and efforts for conservation rearing of at-risk Bombus species.

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