Oocytes were extracted from bovine ovaries by perforation of the follicle wall. Oocytes extracted were chosen for culture based on the cumulus-oocyte complex (COC), graded by the density of cumulus cells on a scale of A-D, in vitro fertilization (IVF) was performed on COC’s rated A or B. Healthy COC were placed into a maturation media for incubation at 29 degree C with a 5 percent CO2 air mixture for twenty four hours. After the maturation period the chosen oocytes were fertilized by IVF using frozen/thawed semen from one bull.
Addley, Tyce and Campbell, Kenneth, "Observation of Fertilized Bovine Oocytes" (2016). Biology Posters. Paper 29.