Transfection of Cystic Fibrosis Sequence into Host Cells

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To better study the effects of Cystic Fibrosis on the human body, it is important to be able to effectively and efficiently recreate the disease in an appropriate mammalian model. New technology in transfection reagent, jetPRIME, hopes to allow us to efficiently transfer these Cystic Fibrosis sequences into sheep embryos to allow the traits to be expressed on a more reliable basis on the cloned sheep.

My research with this reagent was to test the reliability of jetPRIME to transfer our desired DNA sequences into cells at a more efficient rate then previously demonstrated by other reagents. Because of the small number of sheep embryos available for transfection, and the expenses of DNA used for Cystic Fibrosis research, it's important that we achieve the highest efficiency possible. This took many attempts at tranfection with different concentrations of reagent and DNA

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