Cells and Materials


Glow discharge (GD)-treated and autoclaved glass-ceramics of bone-bonding and non-bonding type were implanted into the femoral diaphysis of rats for 3, 7, 14 and 28 days and were investigated by applying light microscopy and histomorphometry , and scanning and transmission electron microscopy. More bone and chondroid, and faster osteoid development were observed at glow-discharge treated implants even in nonbonding implants when compared to autoclaved controls. Ultrastructural investigations showed a higher leach ing- rate of GD-treated bone-bonding implants during the early days after implantation leading to a higher rugosity, whereas no morphological changes were observed on non-bonding implant surfaces. More extracellular matrix (ECM) forming productive cells and less macrophages were observed on GD-sterilized implant surfaces. Osteoclast-like cells were detected exclusively at 3 and 7 days post-operatively on GD-sterilized bone-bonding implants. The higher amount of bone at GD-treated surfaces was thought to be due to a higher leaching rate leading to higher surface-rugosity and interface alkalinity. Thus, GD-treatment seems to be suitable for sterilization of surface-reactive implants to yield earlier functionality and to reduce possible degradative processes.