Cells and Materials


Thin films of hydroxylapatite, about 500 nm, were formed on Ti6Al4V by excimer laser ablation. Ten different deposition conditions were used such as deposition atmosphere (vacuum, pure water vapor, mixture of Ar and water vapor), substrate temperature, target-substrate distance and energy density. Mechanical and physical analyses were carried out by Rutherford backscattering and particle induced X-ray emission; crystallinity of films was determined by X-ray diffraction, surface topography by scanning electron microscopy and adhesion by a scratch test. Biological evaluation of samples was also performed. The fibroblast proliferation test was used, morphological evaluation of macrophages was carried out, and the activity of lymphocytes cultured in the presence of implants was observed.