Cells and Materials


The tissue/biomaterial interactions of three biomaterials of potential use in bone implants were studied in vitro. The mechanism of cell adherence to various ceramic substrata has been investigated by measurement of short term (90 minutes) cell attachment and spreading rate. We have determined the effect of two serum glycoproteins, fibronectin and vitronectin, on the adhesion of human bone derived cells (HBD-cells) cultured on three different types of ceramics {alumina (Al2O3), hydroxyapatite (HAP) and yttria-doped tetragonal zirconia polycrystal (Y-TZP)} . The attachment of HBD-cells to alumina and hydroxyapatite was approximately 60% of that to Y-TZP. Furthermore, the requirement for serum adhesive glycoproteins vitronectin and fibronectin, for HBD-cell attachment to Al2O3, HAP and Y-TZP reveals a dependence upon serum vitronectin for the initial attachment of HBD-cells. There was no difference in the mechanism of initial adhesion between bioactive hydroxyapatite, as compared to the "bioinert" ceramics, alumina and Y-TZP, so the mechanism of the initial attachment of HBD-cells appears not to be dependent on the ceramic composition. The effect of surface roughness of alumina in the order of 8.3-70.7 nm on the adhesion of HBD-cells was also investigated. The Al2O3 disks with a root mean square surface roughness (roughness assessment: Ra) value of 8.3 nm had significantly fewer cells attached than those with an Ra of 70.7 nm.

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