Cells and Materials


It is well known that cell behaviors such as adhesion, proliferation and various synthesis are initiated from transmembrane signals. This study uses biomaterials as primary messengers of the cell activation pathways, and we have analyzed the effects of two biomaterials on highly metastatic tumor cells. B16F10 melanoma cells formed heterogeneous populations whose size varied with cell differentiation. In long-term organ cultures grown comparatively on AN 69 and Cuprophan (a biomembrane known to activate cells), we found that Cuprophan increased both adhesion and proliferation of small melanin-rich cells which represented differentiated melanocyte&. In dissociated cell cultures, the rate of early cell attachment decreased on Cupropban compared to AN 69 and control Thermanox® (Nunc Inc., Naperville, IL). Scanning electron microscopy of melanocytes four hoUl11 after plating out on Cuprophan revealed only cell aggregates, comparable to the 3T3 fibroblasts aggregates previously described. Nevertheless, the production of the second messenger cyclic adenosine monophosphate (cAMP) was the same on both materials, in contrast to previous results showing more cAMP in cells on Cuprophan. Therefore, biomaterials appear to be useful tools for investigating as well attachment, growth, differentiation as signal transduction pathways of cancerous cells.