Impact of basal diet on obesity phenotype of recipient mice following fecal microbiome transfer from obese or lean human donors. Appendix D.  Microbiome sequencing data

Authors:
Daphne Rodgriguez, daphne.rodriguez@aggiemail.usu.edu
Abby Benninghoff, abby.benninghoff@usu.edu
Korry Hintze, Korry.hintze@usu.edu

The complete data set generated by 16S rRNA sequencing of all fecal samples analyzed is provided as a digital data .biom file accessible. ).  Biom files can be read using Qiime software, which is freely accessible at Qiime.org.   A mapping file is also provided as a tab-delimited .txt document, and Table D.1 provides a description of the mapping file content according to the column title.  

TABLE D1.  
Definitions of file contents for Appendix D by column title
SampleID - Numeric sample ID
BarcodeSequence - Barcode sequence for library preparation to identify samples post sequencing
LinkerPrimer: Linker primer sequence
ReversePrimer	 - Reverse primer sequence
MID - Barcod primer ID
Study - Letter designating the experimental study by our research group; ÒHÓ refers to the study data included in the associated .biom file
Timepoint - Time points designated as follows: 1, initial; 2, post fecal microbiome transfer; 3, terminal
BodyType - Body type of the human fecal microbiome transfer donor: L, lean human donor; O, obese human donor
Donor - Numeric ID number corresponding to human donors
Diet - Experimental diet provided to recipient mice:  A, AIN diet; D, DIO diet; T, TWD diet 
Mouse - Numeric ID corresponding to individual mouse within each experimental group
Description - Complete sample code, in the following order, Study.Timepoint.BodyType.Donor.Diet.Mouse