1. Dataset Title: Energy expenditure across immune challenge severities
in a lizard: consequences for innate immunity, locomotor performance and oxidative status




2. Name and contact information of PI: 


a. Name: Susannah French


b. Institution: Utah State University


c. Address: 5305 Old Main Hill, Logan Utah 84322-5305


d. Email: susannah.french@usu.edu


e. ORCiD ID: 0000-0001-8923-9728 


3. Name and contact information of Co-PI: 


a. Name: Spencer Hudson


b. Institution: Utah State University


c. Address: 5305 Old Main Hill, Logan Utah 84322-5305


d. Email: spencer.hudson@usu.edu


e. ORCiD ID: 0000-0003-1806-8188



3. Funding source (Agency, Grant Number) if applicable: 



National Science Foundation, IOS 1350070




4. Project summary, description or abstract:


Reptiles, like other vertebrates, rely on immunity to defend themselves from infection. The energetic cost of an immune response is liable to scale with infection severity, prompting constraints on other selfmaintenance traits if immune prioritization exceeds energy budget. In this study, adult male side-blotched lizards (Uta stansburiana) were injected with saline (control) or high (20 μg g−1 body mass) or low (10 μg g−1 body mass) concentrations of lipopolysaccharide (LPS) to simulate bacterial infections of discrete severities. The costs and consequences of the immune response were assessed through comparisons of change in resting metabolic rate (RMR), energy metabolites (glucose, glycerol, triglycerides), innate immunity (bactericidal ability), sprint speed and oxidative status (antioxidant capacity, reactive oxygen metabolites). High-LPS lizards had the lowest glucose levels and greatest sprint reductions, while their RMR and bactericidal ability were similar to those of control lizards. Low-LPS lizards had elevated RMR and bactericidal ability, but glucose levels and sprint speed changes between those of high-LPS and control lizards. Levels of glycerol, triglycerides, reactive oxygen metabolites and antioxidant capacity did not differ by treatment. Taken together, energy expenditure for the immune response varies with challenge severity, posing consequences for self-maintenance processes in a reptile.


5. Brief description of collection and processing of data: Physiological and morphological performance metrics of side-blotched lizards 


6. Description of files (names, or if too numerous, number of files, file type(s): 



hudsonetal_utaLPSperformance.csv; csv format




7. Definition of acronyms, codes, and abbreviations:


id (identification number), svl.mm (snout-vent length), mass.1.g (pre- body mass)	mass.2.g (post- body mass), mass.delta (change in body mass), treatment (treatment group), mass.adj.dose (LPS dosage per gram body mass,) tb.1.C (body temperature 1), tb.2.C (body temperature 2), baseline.speed.m.s (pre sprint speed), post.speed.m.s (post sprint speed), sprint.delta (change in sprint speed),	total.food.mass.provided.g (total mass of crickets provided), total.food.intake.g (total mass of crickets eaten),	pre.O2.massadj (pre mass adjusted oxygen consumption), post.O2.massadj (post mass adjusted oxygen consumption),	bka.% (plasma bactericidal ability), glu.mg.dl	(plasma glucose), tri.mg.dl (plasma triglycerides), gly.mg.dl (plasma free glycerol), droms.mg.h2o2.dl (plasma reactive oxygen metabolites), oxy.mol.hclo.ml (plasma antioxidant capacity)
 

8. Descriptions of parameters/variables

a. Temporal (beginning and end dates of data collection): 16 – 25 April 2019


b. Instruments used and units of measurements: 
Custom-made racetrack measuring sprint speeds in meters per second
Scale measuring mass in grams
Metric ruler measuring snout-vent-length in millimeters
Photospectrometer to measure bactericidal ability, free glycerol, triglycerides, reactive oxygen metabolites (mg H2O2/dl) and antioxidant capacity (mol HClO/ml)
Point of care device to measure plasma glucose in milligrams per deciliter


c. Column headings of data files (for tabular data): 

id	svl.mm	mass.1.g	mass.2.g	mass.delta	Treatment	mass.adj.dose	tb.1.C	tb.2.C	baseline.speed.m.s	post.speed.m.s	sprint.delta	total.food.mass.provided.g	total.food.intake.g	pre.O2.massadj	post.O2.massadj	bka.%	glu.mg.dl	tri.mg.dl	gly.mg.dl	droms.mg.h2o2.dl	oxy.mol.hclo.ml


d. Location/GIS Coverage (if applicable to data): 38.07° latitude, -109.56° longitude, 1559 m elev.


e. Symbol used for missing data: NA


9. Special software required to use data: No


10. Publications that cite or use this data: Lopes, P. C., French, S. S., Woodhams, D. C., & Binning, S. A. (2021). Sickness behaviors across vertebrate taxa: Proximate and ultimate mechanisms. Journal of Experimental Biology, 224(9), jeb225847. https://doi.org/10.1242/jeb.225847



11. Was data derived from another data source?  If so, what source?: No