Scanning Electron Microscopy


Sensory neurons of 8-week human fetal dorsal root ganglia were dissociated into single cells by trypsinization and cultured on coverslips for 4 months, in either serum-containing or serum-free chemically defined media. At different times the cultures were fixed and prepared for scanning electron microscopy. Fetal sensory neurons in culture regenerated axons within 24 hours which were characterized by axonal growth cones at their tips and the neuronal perikarya assumed spherical or a bonnet-like morphology.

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