Single intact frog skeletal muscle fibers quick-frozen after known time intervals following electrical stimulation are examined electron microscopically in thin sections, after freeze-substitution, in freeze-fracture/etch preparations, and in cryosections prepared for x-ray microprobe analysis. Techniques are described to perform these operations on a single fiber. Notable morphological differences between conventionally fixed and cryopreserved muscle fibers, and between fibers quick- frozen at different post-stimulation intervals are demonstrated.
Nassar, Rashid; Wallace, Nancy R.; Taylor, Isaiah; and Sommer, Joachim R.
"The Quick-Freezing of Single Intact Skeletal Muscle Fibers at Known Time Intervals Following Electrical Stimulation,"
Scanning Electron Microscopy: Vol. 1986
, Article 33.
Available at: https://digitalcommons.usu.edu/electron/vol1986/iss1/33