Eggs of Trichuris trichiura were prepared for scanning electron microscopy (SEM) by the dimethylsulfoxide freeze-cracking method. The egg-shell and oocyte were examined by SEM. The egg has a chitinous shell which consists of more than 10 layers of dense lamellae. The shell is bordered by a limiting membrane. An operculum and a collar made of chitinous shell together form the opercular area. The operculum is an empty cavity. The chitinous fibers of the egg-shell in this area are diffuse and loose, with numerous micropores or spaces. The egg-shell in this area therefore appears to form a fine tubular network. The oocyte is an undifferentiated cell with a biconcave drum-like shape. The perivitelline space is conspicuous at both ends of the cell.
Meng, Xianqin; Wang, Songshan; Zhou, Wenqin; Wang, Boxia; Han, Wensu; and Wang, Li
"The Operculum-Plug Area and Membranous Structure of the Eggs of Trichuris Trichiura,"
Scanning Electron Microscopy: Vol. 1986
, Article 20.
Available at: https://digitalcommons.usu.edu/electron/vol1986/iss3/20