Scanning Electron Microscopy


A fibrous band of extracellular materials on the chick embryo area pellucida/area opaca border is a preferential migratory pathway for chick embryo primordial germ cells (PGC). This band contains fibronectin, collagen Type I and sulphated glycosaminoglycans. It is known that PGCs from Xenopus laevis interact with fibronectin as they undergo migration in the embryo from their site of origin to the gonads. To establish whether this pathway is species specific in chick embryos it was decided to transplant PGC from Xenopus laevis embryos stage 48 on to chick embryos stage 4 fibrous band. Their rapid migration on this extracellular matrix and their subsequent re-orientation of the basement membrane has been studied by scanning electron microscopy.

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