To Resin or Not to Resin in Immunocytochemistry

Authors

J. J. Wolosewick, University of Illinois at the Medical Center

Abstract

Hydrated resinless sections produced by a variety of methods (cryoultramicrotomy or polyethylene glycol techniques) appear to be excellent specimens for post-embedding immunocytochemistry at the electron microscopic level. A perplexing problem is the lack of apparent penetration throuqhout the section thickness of particulate probes such as ferritin or colloidal gold. This report draws attention to the possible causes for this phenomenon namely the size of the probe and the possible effects of fixation or processing. Smaller probes, gentler fixation or permeabilization procedures and increased incubation times all seem to be logical approaches to increasing penetration of immunoreagents into thick hydrated sections.

Recommended Citation

Wolosewick, J. J. (1985) "To Resin or Not to Resin in Immunocytochemistry," Scanning Electron Microscopy: Vol. 4: No. 1, Article 25.
Available at: https://digitalcommons.usu.edu/electron/vol4/iss1/25