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Transient transfection is a method to introduce foreign DNA into a healthy cell population. Transient transfection can be accomplished by chemical, mechanical, and electrical delivery. Although foreign DNA is not incorporated into the host cell genome, host cell machinery is used to transcribe and translate the gene of interest. This presentation will illustrate the enhancement of transgene production through reagent optimization and feeds and show benefits of transient work, such as, eliminating the need for generation of a stable engineered cell line; allowing the process scaleability; and decreasing the time for generation of comparable amounts of product.
Utah State University
process, optimization, enhance, quality, quantity, transiently, proteins, biological, engineering
USU College of Engineering, "Process Optimization to Enhance Quality and Quantity of Transiently Expressed Proteins | Biological Engineering" (2015). College of Engineering News. 39.