Date of Award:

5-2014

Document Type:

Thesis

Degree Name:

Master of Science (MS)

Department:

Computer Science

Advisor/Chair:

Nicholas S. Flann

Abstract

As stoichiometric metabolic models increase in complexity and delity, design and reconstruction tools are urgently needed to increase the productivity of this time-consuming process. Engineers require software for the exploration, evaluation, and rapid analysis of model alternatives within an intuitive visualization and data management framework. This thesis introduces such a tool: Pathway Pioneer (www.pathwaypioneer.org), a web based system built as a front-end graphical user interface to the ux balance analysis tool COBRA. Pathway Pioneer adds additional functionality for customized network layout and model-engineering collaboration through shared models and model version control. Pathway Pioneer is a dynamic, clickable, browser-based visualization system for metabolic network models retrieved from databases such as BiGG or developed in-house as SBML or XLS compliant les. The user can customize the network layout to visually organize the metabolites and reactions into functional modules. The tool supports zooming and panning, level-of-detail control, ux visualization, keyword searching, and hierarchical subsystem organization. A reaction may be knocked out, set as an objective, looked up in a database or many other operations by a single click on the visualized network. Following each operation the visualization is refreshed with the new ux values. The system supports model revision control to manage alternative network congurations and supports sharing of models and layouts to the broader community. By moving the computationally intensive model analysis from the user computer to remote servers, Pathway Pioneer enables the application of high performance cloud-based resources for greater eciency and scalability. I demonstrate the utility of Pathway Pioneer through application in model reconstruction and analysis of many standard models and also two new models under development: Eukaryotic multicompartment Chinese Hamster Ovary (Cho) cells and in a large-scale Escherichia coli system for bio-manufacturing.

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