Title

Membrane Protein as a Basis of NACL Tolerance in Alfalfa

Author

Husni N. Sabah, Utah State University

Date of Award:

5-1995

Document Type:

Thesis

Degree Name:

Master of Science (MS)

Department:

Plants, Soils, and Climate

Committee Chair(s)

W. F. Campbell

Committee

W. F. Campbell

Committee

J. J. Jurinak

Committee

G. A. Long

Abstract

This study sought to determine whether NaCl altered the plasma membrane proteins in alfalfa exhibiting differential NaCl concentrations, and whether CaSO₄ modified the responses. Two alfalfa cultivars, Centurion and Condor, were grown in 0.5 strength Hoagland solution in a greenhouse. The cultivars were exposed to 0, 88, and 132 mM of NaCl alone and mixed with CaSO₄ ⋅ H₂O at 7 and 14 Mm CaSO₄ for 3, 9, and 60 days.

In experiment 1, roots were dried to determine their Na, Ca, K, and Mg concentration. The results were similar to previous reports in which CaS04 alleviated the salt stress by increasing K and Mg levels and reducing Na.

In experiment 2, after proteins of the plasma membrane were isolated and their purity was determined by vanadate, ATPase activity showed a significant increase in the presence of calcium. In addition, total plasma membrane protein was analyzed by sodium dodecylsulfate-polyacrylamide gel electrophoresis. Salt treatments induced both quantitative and qualitative changes in proteins. These changes were affected by the length of exposure to treatment solution or the ability of the plants to adapt to the salt stress.

Checksum

d88a783147595914bb1109f43fa7f0d6

Recommended Citation

Sabah, Husni N., "Membrane Protein as a Basis of NACL Tolerance in Alfalfa" (1995). All Graduate Theses and Dissertations. 3414.
https://digitalcommons.usu.edu/etd/3414