Date of Award:
Master of Science (MS)
Plants, Soils, and Climate
William F. Campbell
The objective of this study was to identify DNA markers for each of six added C-genome chromosomes in Triticum aestivum L. cv. 'Alceso'-Aegilops caudata L. addition lines using the randomly amplified polymorphic DNA (RAPD) technique. DNA from Ae. caudata, T. aestivum, amphiploid of T. aestivum X Ae. caudata, and six disomic addition lines of wheat having a pair of Ae. caudata chromosomes was used as the template for the amplification of RAPD markers with a total of 58 random 10-mer oligonucleotide primers. Two primers, OPC-08 and OPJ-16, produced one intense band each from the amphiploid of T. aestivum X Ae. caudata and Ae. caudata, which was absent in all six addition lines. Each of these two primers produced a chromosome marker that could be tentatively located to the chromosome CA of Ae. caudata. OPJ-02, OPD-12, OPD-02, OPJ-12, OPD-20, and OPJ-14 produced a marker each for CB, CC, CD, CE, CF, and CG, respectively. OPJ-09 produced C-genome chromosome-specific RAPD markers. Also, OPC-05 and OPJ-19 produced RAPDs from both wheat and Ae. caudata genomes.
Wei, Ling, "Identification of DNA Markers in Triticum aestivum-Aegilops caudata Additions Lines by Randomly Amplified Polymorphic DNA (RAPD) Technology" (1995). All Graduate Theses and Dissertations. 4543.
Copyright for this work is retained by the student. If you have any questions regarding the inclusion of this work in the Digital Commons, please email us at .