Date of Award:
Master of Science (MS)
Randolph V. Lewis
Spider aggregate glue is secreted on to the webs of many spiders in the superfamily Araneoidea. Aggregate glue is sticky when wet and has a unique stress strain curve that varies depending on the relative humidity and speed of probe retraction. These features make it an attractive target for applications such as underwater adhesives or surgical glues. However, little is known about the genetic sequence of the glue protein itself. In this work, a method is identified to isolate the remainder of the aggregate gene sequence, and genetic constructs glue are created using known aggregate gene sequence. One construct is expressed in E. coli BL21 cells and the protein is tested for its adhesive properties.
Berg, Kyle, "Spider Aggregate Glue Sequence Characterization and Expression" (2016). All Graduate Theses and Dissertations. 5022.
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