Date of Award:

2016

Document Type:

Thesis

Degree Name:

Master of Science (MS)

Department:

Biology

Advisor/Chair:

Randolph V. Lewis

Abstract

Spider aggregate glue is secreted on to the webs of many spiders in the superfamily Araneoidea. Aggregate glue is sticky when wet and has a unique stress strain curve that varies depending on the relative humidity and speed of probe retraction. These features make it an attractive target for applications such as underwater adhesives or surgical glues. However, little is known about the genetic sequence of the glue protein itself. In this work, a method is identified to isolate the remainder of the aggregate gene sequence, and genetic constructs glue are created using known aggregate gene sequence. One construct is expressed in E. coli BL21 cells and the protein is tested for its adhesive properties.

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004d8209623d1178e210129bdb50bf90

Included in

Biology Commons

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