Date of Award:
5-1972
Document Type:
Thesis
Degree Name:
Master of Science (MS)
Department:
Nutrition, Dietetics, and Food Sciences
Department name when degree awarded
Nutrition and Food Sciences
Committee Chair(s)
Gary H. Richardson
Committee
Gary H. Richardson
Committee
C. A. Ernstrom
Committee
J. C. Batty
Committee
R. C. Anderson
Abstract
The Technicon AutoAnalyzer II was evaluated for automatic quantitation of non-casein protein (NCP) in milk. The Lowry method for colorimetric measurement of proteins was adapted to the automated method and found to be accurate in the determination of non-casein protein in milk. The automated Lowry method obeyed Beer's law. Casein content was calculated by difference between total protein determined by infrared milk analysis (IRMA) and non-casein protein. In the analysis of twenty-five replicate milk samples, the standard deviation was 0.32 and the coefficient of variability was 0.90. The casein content as a percentage of total protein in the milk of cows in the Utah State University dairy herd was found to vary from 41.0 to 81.3%. The automated or continuous flow method was affected by as little as 0.05% (w/w) mercuric chloride, a chemical preservative used in milk, whereas potassium dichromate and formaldehyde had insignificant effects upon the assay.
Checksum
916bf819ed31d1667b39889761daaa78
Recommended Citation
White, Robert Steven, "Continuous Flow Analysis of Non-Casein Protein in Milk" (1972). All Graduate Theses and Dissertations, Spring 1920 to Summer 2023. 5124.
https://digitalcommons.usu.edu/etd/5124
Included in
Copyright for this work is retained by the student. If you have any questions regarding the inclusion of this work in the Digital Commons, please email us at .