Date of Award:
5-1973
Document Type:
Thesis
Degree Name:
Master of Science (MS)
Department:
Nutrition, Dietetics, and Food Sciences
Department name when degree awarded
Nutrition and Food Sciences
Committee Chair(s)
Gary H. Richardson
Committee
Gary H. Richardson
Abstract
A substrate was developed for measuring the milk clotting strength of rennet preparations on an automatic clot-timer. The substrate contained 8 percent pasteurized skim milk solids, 1 percent chloroform, 0.3 percent 200 bloom gelatin, 0.03 M added CaCl2 and was buffered to pH 6.6 with 0.057 M cacodylic acid and 0.042 M triethanolamine. The substrate was shelf-stable for 18 days at room temperatures. It was found that rennet preparations could be standardized to within 1 percent of each other, in terms of milk clotting strength, by use of the substrate on the automatic clot-timer. The method appears to have advantages over conventional rennet standardizing procedures. The research included studies on the effect of chloroform, nonfat dry milk and CaCl2 concentrations; heat and ionizing radiation on the substrate coagulation time.
Checksum
4a65fc0928c3c4a6a1972143418711c5
Recommended Citation
Stevens, Mark Brimhall, "The Development of an Improved Milk Substrate for Rennet Coagulation Assay on an Automatic Clot-Timer" (1973). All Graduate Theses and Dissertations, Spring 1920 to Summer 2023. 5129.
https://digitalcommons.usu.edu/etd/5129
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