Date of Award:

5-1975

Document Type:

Thesis

Degree Name:

Master of Science (MS)

Department:

Nutrition, Dietetics, and Food Sciences

Department name when degree awarded

Nutrition and Food Sciences

Committee Chair(s)

D. K. Salunkhe

Committee

D. K. Salunkhe

Committee

D. G. Hendricks

Committee

V. T. Mendenhall

Committee

J. Y. Do

Abstract

Anthocyanin pigments of sour cherry (prunus cerasus L., cultivar Montmorency) fruit were studied by spectrophotometry, paper and two-dimensional thin layer chromatography and disc electrophoretic method. Mature and wholesome fresh sour cherries and freeze-dried compressed sour cherries stored at 70 F and 100 F for 6 months were used.

A total of seven anthocyanin pigments were observed in both paper and thin layer chromatograms of the fresh and freeze-dried compressed samples stored for O month whereas the freeze-dried compressed samples stored at 70 F and 100 F for 6 months showed the retention of three to six pigments. All of those seven pigments were unstable and cyanidin-3- (2G- xylosylrutinoside) was the least stable pigment.

The separation of anthocyanina by disc gel electrophoresis was studied for the first time. Disc electropherograms of fresh and freeze-dried com-pressed sour cherries stored at O month revealed the presence of eight bands whereas the freeze-dried compressed sour cherries stored at 70 F and 100 F for 6 months showed the retention of three to four bands. In further study, this technique could be helpful for the separation of anthocyanins in other fruits.

The study indicated that the fresh samples had anthocyanin content higher than those of the freeze-dried compressed samples stored at 70 F and 100 F for 6 months and also showed that the degradation of anthocyanins is greater at the higher storage temperature with longer storage periods.

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