Date of Award:

8-2020

Document Type:

Thesis

Degree Name:

Master of Science (MS)

Department:

Biological and Irrigation Engineering

Committee

Charles D. Miller

Committee

Jixun Zhan

Committee

Ronald C. Sims

Abstract

To improve the cost of producing protein therapeutics manufacturers can attempt to increase the amount of protein produced per unit volume. Some small fatty-acids have shown the ability to increase protein concentrations in some manufacturing systems. An example system is in the industrially important cell line derived from a Chinese Hamster Ovary (CHO). A drawback of adding these fatty-acids is they can increase the rate of cell death.

A potential way to minimize the cell death rate, caused by adding fatty-acids, is to also add additional anti-oxidants. Through a series of studies, we demonstrate production with different fatty-acids, butanoic acid and pentanoic acid, with the addition of the antioxidant, n-Acetyl cysteine (NAC).

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421dea34cd304c3bbdeefad185899b7c

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