Date of Award:

8-2020

Document Type:

Thesis

Degree Name:

Master of Science (MS)

Department:

Biological and Irrigation Engineering

Advisor/Chair:

Charles D. Miller

Co-Advisor/Chair:

Jixun Zhan

Third Advisor:

Ronald C. Sims

Abstract

To improve the cost of producing protein therapeutics manufacturers can attempt to increase the amount of protein produced per unit volume. Some small fatty-acids have shown the ability to increase protein concentrations in some manufacturing systems. An example system is in the industrially important cell line derived from a Chinese Hamster Ovary (CHO). A drawback of adding these fatty-acids is they can increase the rate of cell death.

A potential way to minimize the cell death rate, caused by adding fatty-acids, is to also add additional anti-oxidants. Through a series of studies, we demonstrate production with different fatty-acids, butanoic acid and pentanoic acid, with the addition of the antioxidant, n-Acetyl cysteine (NAC).

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