Date of Award:
8-2020
Document Type:
Thesis
Degree Name:
Master of Science (MS)
Department:
Biological Engineering
Committee Chair(s)
Charles D. Miller
Committee
Charles D. Miller
Committee
Jixun Zhan
Committee
Ronald C. Sims
Abstract
To improve the cost of producing protein therapeutics manufacturers can attempt to increase the amount of protein produced per unit volume. Some small fatty-acids have shown the ability to increase protein concentrations in some manufacturing systems. An example system is in the industrially important cell line derived from a Chinese Hamster Ovary (CHO). A drawback of adding these fatty-acids is they can increase the rate of cell death.
A potential way to minimize the cell death rate, caused by adding fatty-acids, is to also add additional anti-oxidants. Through a series of studies, we demonstrate production with different fatty-acids, butanoic acid and pentanoic acid, with the addition of the antioxidant, n-Acetyl cysteine (NAC).
Checksum
421dea34cd304c3bbdeefad185899b7c
Recommended Citation
Camire, Joseph, "Characterization of Chinese Hamster Ovary Cell Performance with Pentanoic Acid and N-Acetyl Cysteine Treatment" (2020). All Graduate Theses and Dissertations, Spring 1920 to Summer 2023. 7841.
https://digitalcommons.usu.edu/etd/7841
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