Date of Award:
5-1-1975
Document Type:
Dissertation
Degree Name:
Doctor of Philosophy (PhD)
Department:
Biology
Department name when degree awarded
Biochemistry
Committee Chair(s)
Larre N. Egbert
Committee
Larre N. Egbert
Abstract
Electron microscopy, isopycnic centrifugation, thermal denaturation profiles and polyacrylamide gel electrophoresis were used to characterize the nucleic acid from neonatal calf diarrhea virus. The genome was shown to consist of 11 segments of double-stranded RNA. The molecular weights of the segments were determined to be: (in million dalton units) 2.18 ± .06, 1.73 ± .07, 1.64 ± .06, 1.48 ± .06, 0.94 ± .03, 0.77 ± .02, 0.50 ± .03, 0.50 ± .03, 0.50 ± .03, 0.29 ± .03, 0.22 ± .02. Six NCDV capsid polypeptides are described. The molecular weights of the capsid polypeptides ranged from 0.15 x 105-1.27 x 105 daltons. A possible direct coding relationship for the capsid polypeptides was indicated.
Recommended Citation
Barnett, Bill B., "Partial Characterization of the Genome of Neonatal Calf Diarrhea Virus (Reoviruslike Agent)" (1975). Biology. 411.
https://digitalcommons.usu.edu/etd_biology/411
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