Date of Award:
5-1-1980
Document Type:
Thesis
Degree Name:
Master of Science (MS)
Department:
Biology
Department name when degree awarded
Life Sciences:Biology
Committee Chair(s)
Jon Y. Takemoto
Committee
Jon Y. Takemoto
Committee
Elizabeth Boeker
Committee
Frederick Post
Abstract
The purpose of this study was to investigate the protein components of the light harvesting complex of Rhodopseudomonas sphaeroides. Two protein bands, polypeptides 15A and 15B, were isolated by preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis. Molecular weight values of 9,400 daltons for polypeptide 15A and 9,304 daltons for polypeptide 15B were calculated by amino acid composition. Molecular weights were also determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Polypeptide 15A was estimated to have a molecular weight of 8,250 ± 10% daltons from 12% acrylamide gels and 9,350 ± 10% daltons from 15% acrylamide gels. Polypeptide 15B was estimated to have a molecular weight of 7,400 ± 10% daltons from 12% acrylamide gels and 7,050 ± 10% daltons from 15% acrylamide gels. Amino acid composition analysis showed high levels of neutral and nonpolar residues and low levels of cysteine and histidine of both 15A and 15B. Comparison of amino acid compositions of the two proteins showed differences in the levels of serine, alanine, and isoleucine.
Recommended Citation
McMillan, Brent D., "Isolation and Characterization of Light Harvesting Proteins From the Photosynthetic Membranes of Rhodopseudomonas sphaeroides" (1980). Biology. 457.
https://digitalcommons.usu.edu/etd_biology/457
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