Date of Award:
5-1-1980
Document Type:
Thesis
Degree Name:
Master of Science (MS)
Department:
Biology
Department name when degree awarded
Life Sciences:Biology
Committee Chair(s)
Rex S. Spendlove
Committee
Rex S. Spendlove
Abstract
MDBK,LLC-MK2 and Intestinal 407 were the most susceptible cell lines respectively in a comparison study using 12 continuous cell lines and reovirus serotypes 1, 2, and 3 isolated from sewage. In a similar study, MDBK cells, when compared to LLC-MK2 and BGM cells, were the most susceptible to sewage isolated, protamine precipitated reovirus, which had not been serotyped and had no previous cell contact. Sewage-isolated, protamine-precipitated reoviruses were also used in conjunction with MDBK cells in a comparative evaluation of immunofluorescent cell count (ICC) and a plaque assay procedures. The ICC assay detected higher reovirus titers in 18-24 hrs, considerably less time than the plaque assay. Reovirus in excess of 104 /liter of raw sewage was detected by the ICC assay method.
Recommended Citation
Ridinger, David N., "Evaluation of a Sensitive Detection Procedure for Sewage-Isolated Reovirus" (1980). Biology. 459.
https://digitalcommons.usu.edu/etd_biology/459
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