Date of Award:
5-1-1981
Document Type:
Thesis
Degree Name:
Master of Science (MS)
Department:
Biology
Department name when degree awarded
Life Sciences:Biology
Committee Chair(s)
Rex S. Spendlove
Committee
Rex S. Spendlove
Abstract
Cell-culture adapted bovine rotavirus was inactivated by varying exposures to ultraviolet light. The purpose was twofold: (1) to ascertain if efficient recombination would be demonstrated with rotavirus and (2) to determine optimal radiation dosages required to induce lethal mutations while retaining a relatively high efficiency of recombination. When assayed by the immuno-fluorescent cell counting method, the irradiated virus exhibited the phenomenon of multiplicity reactivation, suggesting the existence of a mechanism of genetic recombination. Once it had been determined that the double-stranded genome was suitable for additional genetic studies, a modified plaque assay was employed to screen plaque-purified bovine rotavirus for chemically-induced temperature sensitivity.
Recommended Citation
Syverud, John B., "Primary Genetic Analysis of Bovine Rotavirus" (1981). Biology. 478.
https://digitalcommons.usu.edu/etd_biology/478
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