Date of Award:

5-1-1981

Document Type:

Thesis

Degree Name:

Master of Science (MS)

Department:

Biology

Department name when degree awarded

Life Sciences:Biology

Committee Chair(s)

Rex S. Spendlove

Committee

Rex S. Spendlove

Committee

Robert W. Sidwell

Committee

John Simmons

Committee

Bill B. Barnett

Abstract

By coelectrophoresis in polyacrylamide gels, bovine, simian, and porcine rotaviruses exhibited different migration patterns in their double stranded RNA genome. There was less variation between bovine and simian rotavirus migration patterns than between bovine and porcine rotavirus patterns. Nucleotide sequence homology determined by hybridization between 3H labeled double stranded RNA and in vitro produced single stranded RNA showed 74 percent homology between bovine and simian rotaviruses, and 8 percent homology between bovine and porcine rotaviruses. The lack of a close relationship between bovine and porcine rotaviruses was confirmed by hybridization between 3H labeled, in vitro produced, single stranded RNA and double stranded viral genomic RNA.

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