Date of Award:
5-1-1983
Document Type:
Thesis
Degree Name:
Master of Science (MS)
Department:
Biology
Department name when degree awarded
Life Sciences:Biology
Committee Chair(s)
John R. Simmons
Committee
John R. Simmons
Committee
J. T. Bowman
Committee
R. V. Canfield
Abstract
The study was conducted to induce and characterize cold-sensitive lethal mutations in the Basc stock of Drosophila melanogaster and then to identify the effective lethal phase (LP) and temperature-sensitive period (TSP) for the mutants. Ethyl methanesulfonate was used as the mutagenic agent in this study. Basc EMS-treated males were mated to virgin Canton-S females. The progeny from their mating was individually mated and tested for lethality at 18°C and then for viability at 25°C. The stable stocks obtained were then retested for lethality at 18°C. Two different sex-linked, recessive, cold-sensitive lethal mutants were obtained out of 884 individually tested F1 males. The LP and TSP for both stocks were determined and described. The stocks obtained in this study will be useful in such applications as virginizing methods, selective elimination of heterozygotes, and fine structure studies. Due to the variability and instability of cold-sensitive stocks, care should be taken in using such stocks in such studies.
Recommended Citation
Abu-Tarboush, Faisal M., "Induction and Characterization of Cold-Sensitive, Recessive, Sex-Linked Lethal Mutation in Drosophila melanogaster" (1983). Biology. 495.
https://digitalcommons.usu.edu/etd_biology/495
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